The main goal of this self-study was to determine the extent to which an instructor's pedagogical knowledge and practice would be enhanced though the use of Just-in-Time Teaching. The findings reveal that the JiTT strategy has indeed strengthened many areas of the instructor's pedagogical content knowledge. The JiTT activities allowed the instructor to assess easily the prior understandings of her students so that she could better address any misconceptions or gaps in their science knowledge. The in-class follow-up to each activity also forced the instructor to expand her understanding of instructional methodologies. Finally, the findings reveal that the JiTT strategy has indeed strengthened many areas of the instructor's pedagogical content knowledge.